Help:Plasmid Backbones/Features

    The Registry divides a plasmid into two separate components:
  • the part
  • the plasmid backbone

What are the common features on plasmid backbones?

One part can be used in several different plasmids backbones. It's easy to move a part from one plasmid backbone to another.

Prefix and Suffix

    The Prefix and Suffix flank your part
  • Your part does not include the prefix and suffix
  • The sequence of the plasmid backbone starts with the suffix and ends with the prefix

Replication Origin

    Responsible for replicating plasmids during cell growth and division.
  • the plasmid copy number per cell
  • mechanism of copy number control
  • cell-to-cell copy number variation
  • ...and even the degree of coiling of the physical DNA.

Antibiotic Resistance

    Operate your part in the plasmid backbone that works for your system
  • Maybe your part uses a lot of cellular resources, so you'll want to operate it in a low copy plasmid backbone
  • Or you want to test your part in different organisms by using a multi-host plasmid backbone

VF2 and VR sites

    Use a plasmid backbone to measure your part
  • You may want to use a plasmid backbone that will help you measure attributes of your part
  • Have a library of promoters you want to test? Use a plasmid backbone with a built in coding region.
  • Don't forget, documenting and characterizing your part is important!

Learn More About:

Plasmid Backbones: Overview | Shipping in pSB1C3 | Features | Linearized Plasmid Backbones | Plasmid Backbone Catalog
Synthetic Biology | About the Registry | Our Philosophy
Parts | Plasmid Backbones | BioBrick Prefix and Suffix | Standards | Assembly Standards | Assembly Methods